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Peritoneal macrophages during peritonitis. Phenotypic studies
P H Hart1, C A Jones, J J Finlay-Jones
1Department of Microbiology and Infectious Diseases, School of Medicine, Flinders University of South Australia, Adelaide.
Abstract:
The expression of a range of surface molecules/receptors that are important in the host response to infection and foreign antigens was examined using peritoneal macrophages isolated from patients on continuous ambulatory peritoneal dialysis (CAPD) with peritonitis. The macrophage phenotypic profile was compared with that of normal peripheral blood monocytes. Consistently there was increased expression by macrophages of CD14, ICAM-1 (CD54), Fc gamma RI (CD64), Fc gamma RII (CDw32), Fc gamma RIII (CD16), transferrin receptors (CD71) and tissue factor. Increased expression of MHC class II was marginally significant. There was no detectable expression of either the p55 (CD25) or p70 chains of the IL-2 receptor. The expression of the complement receptors, CR1 (CD35) and CR3 (CD11b, CD18), was reduced. The activity of well-known inflammatory cytokines, rather than uraemic molecules, can account for the phenotypic profile of these extravasated peritoneal macrophages. The results of this study indicate that peritoneal macrophages from CAPD patients with peritonitis display a phenotype consistent with them being in vivo-derived inflammatory macrophages, and that they are appropriate for use in studies of anti-inflammatory agents.
Insights
Peritoneal macrophages from patients with continuous ambulatory peritoneal dialysis (CAPD) and peritonitis show increased inflammatory markers. This suggests they are activated in vivo and suitable for anti-inflammatory drug studies.
Area of Science:
- Immunology
- Nephrology
Background:
- Continuous ambulatory peritoneal dialysis (CAPD) patients can develop peritonitis, an infection of the peritoneal membrane.
- Macrophages are key immune cells involved in host defense against infection and foreign antigens.
Purpose of the Study:
- To characterize the surface molecule expression of peritoneal macrophages in CAPD patients with peritonitis.
- To compare the phenotype of these macrophages with normal monocytes.
- To determine if these macrophages are suitable for anti-inflammatory studies.
Main Methods:
- Isolation of peritoneal macrophages from CAPD patients with peritonitis.
- Comparison of macrophage phenotype with normal peripheral blood monocytes using surface molecule/receptor expression analysis.
- Analysis included markers like CD14, ICAM-1, Fc gamma receptors, transferrin receptors, tissue factor, MHC class II, IL-2 receptor chains, and complement receptors.
Main Results:
- Increased expression of CD14, ICAM-1, Fc gamma receptors (CD64, CDw32, CD16), transferrin receptors (CD71), and tissue factor on peritoneal macrophages.
- Marginally significant increase in MHC class II expression.
- No detectable IL-2 receptor chains (CD25, CD70).
- Reduced expression of complement receptors CR1 (CD35) and CR3 (CD11b, CD18).
Conclusions:
- Peritoneal macrophages from CAPD patients with peritonitis exhibit a phenotype consistent with in vivo-derived inflammatory macrophages.
- The observed phenotype is likely driven by inflammatory cytokines rather than uremic toxins.
- These activated macrophages are appropriate for research into anti-inflammatory agents.