Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered lectin binding sites in keratoconus corneas.

W Philipp1

  • 1Department of Ophthalmology, University of Innsbruck, Austria.

Current Eye Research
|May 1, 1992
PubMed
Summary

Keratoconus corneas exhibit altered lectin binding sites, particularly in scarred areas, indicating changes in specific sugar residues. These changes are primarily linked to scar formation, not keratoconus itself.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo confocal microscopic characterisation of the cornea in chronic graft-versus-host disease related severe dry eye disease.

The British journal of ophthalmology·2014
Same author

[Effectiveness of initial antibiotic therapy for treatment of contact lens-related bacterial keratitis].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2013
Same author

Q fever: baseline monitoring of a sheep and a goat flock associated with human infections.

Epidemiology and infection·2012
Same author

First certified reference materials for molecular fingerprinting of two approved probiotic Bacillus strains.

International journal of food microbiology·2008
Same author

A possible loophole in the theorem of Bell.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Bell's theorem and the problem of decidability between the views of Einstein and Bohr.

Proceedings of the National Academy of Sciences of the United States of America·2001

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Glycobiology

Background:

  • Keratoconus is a progressive thinning disorder of the cornea.
  • Glycoconjugates play crucial roles in corneal structure and function.
  • Altered glycoconjugate expression may contribute to keratoconus pathogenesis.

Purpose of the Study:

  • To investigate differences in glycoconjugate expression between normal and keratoconus corneas.
  • To identify specific oligosaccharide structures associated with keratoconus.
  • To determine if observed changes are specific to keratoconus or related to corneal scarring.

Main Methods:

  • Analysis of frozen corneal sections from normal and keratoconus patients.
  • Use of a panel of 12 biotin- or fluorescein isothiocyanate-labeled lectins.
  • Comparative assessment of lectin binding patterns in different corneal layers and scar tissue.

Main Results:

  • No significant differences in lectin binding were observed in the epithelium, endothelium, or Descemet's membrane.
  • Intense peanut agglutinin (PNA) staining was detected in breaks of Bowman's layer and scar tissue in keratoconus corneas.
  • Increased binding for Phaseolus vulgaris erythroagglutinin (PHA-E), Ricinus communis agglutinin I (RCA-I), and Lens culinaris agglutinin (LCA) was noted in scarred regions.

Conclusions:

  • Scarred regions in keratoconus corneas show distinct alterations in glycoconjugate composition, including terminal D-galactose and increased glucose/mannose residues.
  • These alterations are similar to those found in corneal scars from other causes.
  • The study concludes that corneal scar formation, rather than keratoconus itself, is primarily responsible for the observed changes in lectin binding sites.

Related Experiment Videos