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Updated: Aug 10, 2026

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
Published on: November 17, 2011
Portable in situ fluorescence cytometry of microscale cell-based assays
Daniel A Tatosian1, Michael L Shuler, Donghyun Kim
1School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14850, USA.
Abstract:
A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (microCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a microCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the microCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.
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