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An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
[Quantitative analysis of pulegone in Herba Schizonepetae]
Jian-ping Yang1, Bei-hua Bao, Li Zhang
1Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Abstract
Objective:
To establish the determination method of pulegone in Herba Schizonepetae.
Method:
HPLC method was developed for the determination of pulegone in Herba Schizonepetae. The separation was performed on C18 column with MeOH-H2O (80:20) as a mobile phase. The detection wavelength was set at 252 nm.
Result:
The quantitation of pulegone in Herba Schizonepetae varied from 0.2-0.7 mg x g(-1). The average recovery is 96.2%, RSD 0.81%.
Conclusion:
The method is simple, rapid and accurate.
