Chemiluminescence biosensor system for lactic acid using natural animal tissue as recognition element

Area of Science:

• Biotechnology
• Analytical Chemistry
• Biochemistry

Background:

• Lactic acid is a crucial biomarker in various biological and food samples.
• Accurate and rapid detection of lactic acid is essential for clinical diagnostics and quality control.
• Existing methods for lactic acid detection may have limitations in sensitivity, speed, or cost.

Purpose of the Study:

• To develop a novel chemiluminescence biosensor for lactic acid detection.
• To utilize natural animal tissue (porcine kidney) as a recognition element.
• To evaluate the performance and applicability of the developed biosensor.

Main Methods:

• A biosensor was constructed using porcine kidney tissue containing alpha-hydroxy acid oxidase.
• Lactic acid was oxidized to produce hydrogen peroxide, which reacted with luminol and potassium ferricyanide to generate a chemiluminescence (CL) signal.
• The CL signal intensity was measured and correlated with lactic acid concentration.

Main Results:

• The CL emission intensity showed a linear response to lactic acid concentration in the range of 1-1000 micromol/L.
• A low detection limit of 0.2 micromol/L (3sigma) was achieved for lactic acid.
• The biosensor demonstrated continuous usability for 6 hours with over 240 measurements and high reproducibility (RSD < 5%).
• Analysis time per sample was as short as 1.5 minutes with a relative standard deviation of 1.12% for 100 micromol/L lactic acid.

Conclusions:

• The developed porcine kidney-based biosensor is a sensitive, rapid, and stable platform for lactic acid detection.
• The method is suitable for the quantitative analysis of lactic acid in complex matrices like plasma and milk.
• This approach offers a promising alternative for routine lactic acid monitoring.