L5178Y sublines: a look back from 40 years. Part 1: general characteristics

The aim was to review and summarize the results of studies done over the last 40 years concerning the general characteristics and response to ultraviolet C (UV-C) radiation and hydrogen peroxide of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in their sensitivity to various DNA damaging agents. (The response of the sublines to ionizing radiation is described in the second part of the paper.) Comparison of subline karyotypes shows a number of differences in their banding patterns. The sublines differ in their ion transport, the ganglioside pattern of plasma membranes, and in the content and turnover rate of poly(adenosine diphosphoribose) polymers. Nuclear matrix proteins show a differential affinity to these polymers. A unique property of the pair of LY sublines is an inverse cross-sensitivity to X-rays and hydrogen peroxide, with cross-sensitivities to hydrogen peroxide and UV-C, as well as to UV-C and a platinum (Pt) complex (cisplatin analogue). Initial DNA damage and repair and various aspects of the cellular response of the sublines were determined in cells damaged with these agents. The higher sensitivity of LY-R cells to hydrogen peroxide, as compared with LY-S cells, is causally related to the higher content of iron ions in these cells and a less efficient anti-oxidant defence system (including a lower catalase activity). Sensitivity of LY-R cells to UV-C radiation and Pt complexes is explained by impaired excision repair (the incision step is missing).