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WebACT--an online companion for the Artemis Comparison Tool
J C Abbott1, D M Aanensen, K Rutherford
1Centre for Bioinformatics, Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK. admin@webact.org
Bioinformatics (Oxford, England)
|August 4, 2005
Summary
WebACT provides rapid, simultaneous BLAST comparisons for up to five genomic sequences, aiding visualization with Artemis Comparison Tool (ACT). This resource offers on-the-fly and pre-computed comparisons for prokaryotic genomes and plasmids.
Area of Science:
- Bioinformatics
- Genomics
- Comparative Genomics
Background:
- WebACT is an online resource for genomic sequence comparison.
- It facilitates simultaneous BLAST comparisons for multiple sequences.
- Visualizations are compatible with the Artemis Comparison Tool (ACT).
Purpose of the Study:
- To provide a user-friendly platform for rapid genomic sequence comparisons.
- To enable visualization of comparative genomics data using ACT.
- To offer both on-the-fly and pre-computed comparison functionalities.
Main Methods:
- Utilizes simultaneous BLAST comparisons for up to five genomic sequences.
- Integrates with EMBL database queries for sequence retrieval.
- Supports user-provided sequences via direct entry or upload.
- Stores pre-computed comparisons for publicly available prokaryotic genomes and plasmids.
Main Results:
- Enables on-the-fly BLAST comparisons between user-specified or EMBL-retrieved genomic sequences.
- Offers a database of pre-computed comparisons for 372 prokaryotic genomes and plasmids.
- Optimizes data volume and performance for efficient analysis.
- Allows flexible querying of genome sequences, annotations, and comparisons.
Conclusions:
- WebACT enhances comparative genomics by providing rapid, visualized sequence comparisons.
- The platform supports diverse user needs, from custom queries to large-scale genome analysis.
- It serves as a valuable tool for researchers studying prokaryotic genomes and plasmids.
