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Updated: Aug 16, 2026
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
Published on: September 2, 2014
Nonradioactive telomerase activity assay by microchip electrophoresis: privileges to the classical gel
Zhivko Zhelev1, Rumiana Bakalova, Ashraf Ewis
1Single-Molecule Bioanalysis Laboratory (SMBL), National Institute for Advanced Industrial Science and Technology, AIST-Shikoku, Takamatsu, Japan.
Abstract:
The present study accents on the privileges of microchip-based electrophoresis to the conventional gel electrophoresis in separation of telomerase repeat amplification protocol/polymerase chain reaction (PCR) ladder products obtained in telomerase-catalyzed reaction in cancer cells. We try to clarify the interpretation of the results obtained by both electrophoretic procedures and to avoid misinterpretation as a result of PCR-dependent artefacts.
