Related Experiment Video
Updated: Aug 16, 2026
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
Published on: March 4, 2018
[Intracytoplasmic sperm injection using cryopreserved-thawed testicular spermatozoa with testicular fine needle
Xiao-ming Teng1, Li-ping Zhu, Kun-ming Li
1Assistant Reproductive Technology Center, Shanghai First Maternity and Infant Health Hospital, Shanghai 200040, China. tengxm@hotmail.com
Objective:
To evaluate the effect of intracytoplasmic sperm injection (ICSI) using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration (TEFNA) in patients with non-obstructive azoospermia.
Methods:
Sixty-two patients underwent TEFNA. Mature testicular spermatozoa were found in 35 cases of patients and the testicular tissues were cryopreserved for later ICSI. Ovarian stimulation included a long protocol of GnRHa/FSH/hCG. Oocyte retrieval was performed under transvaginal ultrasound guidance, and ICSI conducted with cryopreserved-thawed testicular spermatozoa.
Results:
A total of 35 couples underwent 35 ICSI cycles using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration. The clinical pregnancy rate was 37.14% (13/35).
Conclusion:
ICSI using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration is a main and effective method in the treatment of non-obstructive azoospermia, which can avoid further testicular fine needle aspiration.
