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Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
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Campylobacter jejuni activates mitogen-activated protein kinases in Caco-2 cell monolayers and in vitro infected

Amanda MacCallum1, Graham Haddock2, Paul H Everest1

  • 1Institute of Comparative Medicine, Sir Henry Wellcome Building, University of Glasgow Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QH, UK.

Microbiology (Reading, England)
|August 5, 2005
PubMed

Insights

Campylobacter jejuni infection activates mitogen-activated protein kinases (MAPKs) in human intestinal cells. This suggests MAPKs are key players in the inflammatory response to this common bacterial pathogen.

Area of Science:

  • Cellular biology
  • Immunology
  • Microbiology

Background:

  • Mitogen-activated protein kinases (MAPKs) are crucial signaling proteins in host cellular pathways.
  • MAPKs mediate host responses to bacterial invasion, including cytokine generation and inflammation.

Purpose of the Study:

  • To investigate if Campylobacter jejuni activates MAPKs in the human colon.
  • To understand the role of MAPKs in the inflammatory response to C. jejuni infection.

Main Methods:

  • Infection of Caco-2 cell monolayers (human colonic epithelial cells) with C. jejuni.
  • Infection of human colonic tissue explants with C. jejuni.
  • Assessing MAPK activity (ERK1/2, JNK, p38) and phosphorylation levels.

Main Results:

  • C. jejuni infection significantly increased the activity and phosphorylation of ERK1/2, JNK, and p38 MAPKs.
  • Elevated MAPK activity was observed in both Caco-2 cells and human colonic explants.

Conclusions:

  • Campylobacter jejuni activates key MAPK signaling pathways in human intestinal cells.
  • MAPK activation is implicated in the inflammatory responses triggered by C. jejuni in the human colon.

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