Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of an in vivo bioassay method for allergy-preventive substances using hen-egg white lysozyme

Kyoko Ishiguro1, Hisae Oku, Yoshimi Ueda

  • 1School of Pharmaceutical Sciences, Mukogawa Women's University, Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan. ishiguro@mwu.mukogawa-u.ac.jp

Biological & Pharmaceutical Bulletin
|August 5, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sodium polystyrene sulfonate as an additional contributing factor to repeated gastric ulcers among other multiple factors in a patient undergoing hemodialysis: a case report.

BMC nephrology·2024
Same author

Accelerated involution of germinal center in palatine tonsils in IgA nephropathy.

PloS one·2024
Same author

Anti-Allergic Effects of the Subcritical Water Extract Powder of <i>Citrus unshiu</i> in Mouse and Guinea Pig Models.

Journal of medicinal food·2021
Same author

Atractylodin Produces Antinociceptive Effect through a Long-Lasting TRPA1 Channel Activation.

International journal of molecular sciences·2021
Same author

Effect of polyphenols from <i>Syringa vulgaris</i> on blood stasis syndrome.

Journal of clinical biochemistry and nutrition·2020
Same author

Detoxification Mechanism of α,β-Unsaturated Carbonyl Compounds in Cigarette Smoke Observed in Sheep Erythrocytes.

Chemical & pharmaceutical bulletin·2018

Researchers identified a novel allergy prevention assay by observing decreased vein microcirculation during hen-egg white lysozyme sensitization. This method targets key inflammatory pathways, offering new avenues for allergy treatment development.

Area of Science:

  • Immunology
  • Physiology
  • Pharmacology

Background:

  • Allergic reactions involve complex physiological responses.
  • Hen-egg white lysozyme (HEL) sensitization triggers specific microcirculatory changes.
  • Existing allergy treatments do not fully address early-stage vascular responses.

Purpose of the Study:

  • To discover a phenomenon related to allergic reactions in microcirculation.
  • To develop a new in vivo assay for identifying allergy-preventive substances.
  • To elucidate the molecular pathways involved in HEL-induced microcirculatory changes.

Main Methods:

  • Inducing hen-egg white lysozyme (HEL) sensitization in an animal model.
  • Monitoring blood flow in vein microcirculation without measuring systemic blood pressure.

Related Experiment Videos

  • Testing the efficacy of various antagonists (histamine, serotonin, PAF) and pathway inhibitors (COX, iNOS).

    • Main Results:

    • A significant decrease in vein microcirculation was observed during HEL-sensitization, independent of blood pressure.
    • Antagonists of histamine, serotonin, and platelet-activating factor (PAF) did not alter this blood flow decrease.
    • Cyclooxygenase (COX)-1, COX-2, thromboxane A(2) (TXA(2)), endothelin-1 (ET-1), prostacyclin (PGI(2)), granulocytic elastase (GE), and nitric oxide (NO) from inducible NO synthase (iNOS) were implicated.

    Conclusions:

    • The observed blood flow decrease is mediated by specific inflammatory mediators and pathways.
    • These mediators may contribute to vascular endothelial cell injury during allergic responses.
    • The developed assay effectively identifies potential allergy preventive agents targeting NO, COX-1, COX-2, and PGI(2) pathways.