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Related Experiment Videos

Higher harmonic generation microscopy.

Chi-Kuang Sun1

  • 1Graduate Institute of Electro-Optical Engineering and Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan, ROC. sun@cc.ee.ntu.edu.tw

Advances in Biochemical Engineering/Biotechnology
|August 6, 2005
PubMed
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Higher harmonic generation microscopy offers non-invasive, label-free imaging of live specimens. This technique provides detailed morphological and cellular information without sample preparation or damage, ideal for in vivo studies.

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Nonlinear Optics

Background:

  • Higher harmonic generation (HHG) microscopy, including second harmonic generation (SHG) and third harmonic generation (THG), utilizes virtual-level transitions.
  • This virtual-transition characteristic ensures no energy deposition in the sample, making it a non-invasive imaging modality.
  • HHG microscopy is suitable for in vivo imaging of live specimens without prior preparation.

Purpose of the Study:

  • To highlight the capabilities of higher harmonic generation microscopy for in vivo biomedical imaging.
  • To explain the principles and applications of SHG and THG microscopy.
  • To emphasize the non-invasive and label-free nature of HHG techniques.

Main Methods:

  • Second harmonic generation (SHG) microscopy visualizes organized nano-structures like stacked membranes and proteins via the bio-photonic crystalline effect.

Related Experiment Videos

  • Third harmonic generation (THG) microscopy images cellular or subcellular interfaces, leveraging optical inhomogeneity.
  • HHG microscopy employs nonlinear optical principles for sub-micron 3D sectioning and millimeter penetration.

    • Main Results:

    • HHG microscopy provides label-free imaging of live specimens without energy deposition, saturation, or photobleaching.
    • SHG microscopy reveals structural details in organized biological materials.
    • THG microscopy offers insights into cellular and subcellular interfaces.

    Conclusions:

    • Higher harmonic generation microscopy is a powerful, non-invasive tool for in vivo imaging.
    • It offers label-free morphological, structural, and cellular information without altering the biological environment.
    • The technique enables continuous observation of live specimens, preserving sample viability.