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Updated: Aug 16, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
Published on: March 7, 2022
DHX32 expression suggests a role in lymphocyte differentiation
Mohamed Abdelhaleem1, Tie-Hua Sun, Michael Ho
1Department of Paediatric, Laboratory Medicine, Hospital for Sick Children, University of Toronto, Canada. Mohamed.abdelhaleem@sickkids.ca
Abstract:
RNA helicases constitute a large group of enzymes involved in all aspects of RNA metabolism. Several RNA helicases are dysregulated in cancer, whereas several others are involved in differentiation. DHX32 has previously been identified as a novel RNA helicase with a unique structure and expression pattern. DHX32 message was down-regulated in acute lymphoblastic leukemia cell lines and patient samples. In this report, anti-DHX32 was used to study its expression in thymus. Immunohistochemistry and flow cytometry showed positive correlation of DHX32 expression with thymocyte maturation. These results suggest that DHX32 might play a role in normal lymphocyte differentiation.
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