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Updated: Aug 16, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Gene expression profiling in chemoresistant variants of three cell lines of different origin

Anders Johnsson1, Johan Vallon-Christensson, Carina Strand

  • 1Department of Oncology, University Hospital, S-221 85, Lund, Sweden. Anders.Johnsson@onk.lu.se

Anticancer Research
|August 6, 2005
PubMed
Abstract

Insights

This study reveals key genomic changes in chemotherapy-resistant cancer cells, identifying up-regulated ABC transporters and down-regulated histones. These findings offer a foundation for understanding and overcoming drug resistance in cancer treatment.

Area of Science:

  • Genomics
  • Cancer Biology
  • Pharmacology

Background:

  • Drug resistance is a significant challenge in cancer chemotherapy.
  • Mechanisms of resistance are known, but genomic underpinnings require further elucidation.

Purpose of the Study:

  • To investigate genomic alterations associated with acquired chemotherapy resistance.
  • To identify genes and pathways involved in resistance to cytostatic drugs.

Main Methods:

  • Gene expression profiling using cDNA microarray.
  • Analysis of eight cancer cell lines with acquired resistance to five cytostatic drugs: daunorubicin (DNR), doxorubicin (DOX), vincristine (VCR), etoposide (VP), and mitoxantrone (MX).

Main Results:

  • Resistant cell lines clustered by origin.
  • Significant upregulation of ABC transporter genes (ABCB1, ABCB4, ABCG2).
  • Downregulation of histone genes in resistant MCF-7 sublines; altered expression of GSTs, topoisomerases, caveolins, annexins, and CD44.

Conclusions:

  • Identified specific gene expression changes linked to chemotherapy resistance.
  • Findings provide a basis for further research into resistance mechanisms.
  • Potential for developing strategies to overcome drug resistance in cancer therapy.

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