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p53 intronic G13964C variant in colon cancer and its association with HPV
Nur Buyru1, Ayda Tezol, Nejat Dalay
1I. U. Cerrahpasa Medical Faculty, Department of Medical Biology, Temel Bilimler Binasi, Kat 6 Kocamustafapasa, Istanbul.
Background:
Inactivation of p53 is the most common change identified in human cancer. Nucleotide alterations in p53 intron 6 have been reported to be associated with the dysregulation of p53 function and tumor development. The aim of our study was to assess whether the intron 6 G13964C variant of the p53 gene is associated with the presence of human papillomavirus (HPV) as a risk factor in colon cancer.
Materials And Methods:
To determine whether the intronic G13964C alteration is involved in colon carcinogenesis, surgical specimens from 55 patients with colon cancer and HPV-positive tumors were examined by PCR-RFLP. Blood samples from 77 healthy subjects were used as the control group.
Results:
The 13964C variant was found in 14.5% of colon cancer patients and 12.9% of the control subjects. One patient and two controls were homozygous for this base change.
Conclusion:
No evidence of a significant association between the p53 G13964C allele and colon carcinoma was found.
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