Related Experiment Video
Updated: Aug 16, 2026
An Efficient Method for Adenovirus Production
Published on: June 10, 2021
[Construction of adeno-associated virus vector containing human papilloma virus 16 E7 gene and its expression in
Juan Zhang1, Zhong-qiu Lin, Yong-pai Peng
1Department of Gynecology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Objective:
To construct recombinant adeno-associated virus vector containing human papilloma virus (HPV) 16E7 gene and identify its effectiveness of expression in eukaryotic cell.
Methods:
Recombinant adeno-associated virus particles containing HPV16E7 gene were generated by co-transfection of plasmids pAAV-MCS-E7, pAAV-RC and pAAV-helper into HEK293 cells. The viral particles were collected and purified. The vector titer was measured by southern blot. After the eukaryotic cells were transfected by virus particles, RT-PCR and western blot analysis were performed to identify rAAV expression.
Results:
The recombinant adeno-associated virus vector containing HPV16E7 gene was correctly constructed. The vector titer measured by southern blot was approximately 1 x 10(11)/ml. After the eukaryotic cells were transfected by the recombinant adeno-associated virus vector, the expression of HPV16E7 gene was identified by RT-PCR and western blot.
Conclusions:
Recombinant adeno-associated virus HPV16E7 vector is successfully constructed and can stably express in eukaryotic cells.
