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Published on: November 25, 2009
Development of a shear force scanning near-field fluorescence microscope for biological applications
1LMEN, (EA), UFR des Sciences, Université de Reims Champagne-Ardenne, 21 rue Clément Ader, 51685 Reims Cédex 2, France.
Abstract:
In this paper, a shear force scanning near-field fluorescence microscope combined with a confocal laser microspectrofluorometer is described. The shear force detection is realized based on a bimorph cantilever, which provides a very sensitive, reliable, and easy to use method to control the probe-sample distance during scanning. With the system, high-quality shear force imaging of various samples has been carried out. Furthermore, simultaneous shear force and near-field fluorescence imaging of biological cells has also been realized. As an example, we especially present the result on the distribution of P-glycoprotein in the plasma membrane of human small cell lung cancer cells, suggesting that the system would be a promising tool for biological applications.
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