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Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Improved sensitivity of human immunodeficiency virus type 2 subtype B plasma viral load assay
Florence Damond1, Gilles Collin, Diane Descamps
1Laboratoire de Virologie, Hôpital Bichat Claude Bernard, 46 rue Henri Huchard, 75018 Paris, France. florence.damond@bch.ap-hop-paris.fr
Abstract:
We developed a new assay for human immunodeficiency virus type 2 plasma RNA quantification based on a previous format. The new version performed significantly better than the original regarding the detection of subtype B, allowing the detection of 14 out of 36 plasma RNAs in the subtype B-infected patients not detected with the original version.
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