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Primers for clinical detection of Paracoccidioides brasiliensis
Gioconda San-Blas1, Gustavo Niño-Vega, Laura Barreto
1Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela. sanblasg@ivic.ve
Journal of Clinical Microbiology
|August 6, 2005
Abstract:
From a 0.72-kb fragment universally generated in Paracoccidioides brasiliensis strains, primers were designed and tested on genomic DNA of this and other pathogenic fungi. They were specific and highly sensitive for P. brasiliensis DNA. Positive results were obtained when these were tested in clinical samples.