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Related Experiment Videos

How does yeast respond to pressure?

P M B Fernandes1

  • 1Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brasil. pmbfernandes@gmail.com

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|August 6, 2005
PubMed
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High hydrostatic pressure (HHP) affects Saccharomyces cerevisiae by altering proteins and cell membranes, inducing stress responses and cell cycle arrest. Pretreatment can confer tolerance, but nitric oxide signaling is key for baroprotection.

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Saccharomyces cerevisiae is a model organism for eukaryotic stress response studies.
  • High hydrostatic pressure (HHP) is a significant stressor affecting yeast cell integrity and function.
  • Understanding HHP effects is crucial for its biotechnological applications.

Purpose of the Study:

  • To review the effects of HHP on Saccharomyces cerevisiae.
  • To explore the mechanisms of barotolerance and stress response in yeast.
  • To highlight the role of signaling pathways in HHP adaptation.

Main Methods:

  • Analysis of cellular morphology and viability under varying HHP.
  • Investigation of barotolerance acquisition through stress preconditioning.

Related Experiment Videos

  • Genome-wide expression analysis to identify stress-responsive genes.
  • Assessment of nitric oxide's role in HHP protection.

    • Main Results:

    • HHP induces protein alteration, lipid bilayer changes, and cell cycle arrest in yeast.
    • Morphology remains unaffected below 50 MPa, but cell death occurs above 220 MPa.
    • Acquired barotolerance requires re-incubation at room pressure after HHP pretreatment.
    • Gene expression analysis reveals upregulation of stress defense and carbohydrate metabolism genes, and downregulation of cell cycle and protein synthesis genes.
    • Nitric oxide confers baroprotection, with induced nitric oxide synthase expression under HHP.

    Conclusions:

    • HHP triggers a complex stress response in Saccharomyces cerevisiae involving significant metabolic and regulatory adjustments.
    • Nitric oxide signaling is a critical factor in yeast's ability to withstand high hydrostatic pressure.
    • Further elucidation of pressure-response signaling pathways is necessary for optimizing HHP applications in biotechnology.