Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA)
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Updated: Aug 16, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
Wei Shao1, Hua-Jiang Wei, Ji-Ying Qiao
1Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Abstract:
Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.
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