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Matrix metalloproteinases participate in osteosarcoma invasion
Kristin Bjørnland1, Kjersti Flatmark, Solveig Pettersen
1Institute for Surgical Research, Rikshospitalet, 0027 Oslo, Norway. kristin.bjornland@medisin.uio.no
The Journal of Surgical Research
|August 9, 2005
Summary
Matrix metalloproteinases (MMPs) are involved in osteosarcoma (OS) invasion. MMP inhibitors reduced OS cell invasion, supporting their role in this aggressive bone cancer.
Area of Science:
- Oncology
- Biochemistry
- Molecular Biology
Background:
- Osteosarcoma (OS) is a prevalent, highly malignant bone tumor in children and adolescents.
- Metastasis and relapse in OS patients lead to poor prognosis.
- Enhanced matrix metalloproteinases (MMPs) expression is observed in various solid tumors.
Purpose of the Study:
- To investigate the role of MMPs in human OS cell lines, xenografts, and biopsies.
- To evaluate the potential of MMP inhibitors in controlling OS invasion.
Main Methods:
- Zymography and Northern blot analyses were used to study MMP expression.
- In vitro invasion assays with OS cell lines were performed.
- The effects of MMP inhibitors (Marimastat, doxycycline) on OS cell invasion were assessed.
Main Results:
- Higher MMP-2 and MMP-9 secretion correlated with increased invasiveness in OS cell lines.
- MMP inhibitors significantly reduced OS cell invasion in vitro.
- MMP-2 and MMP-9 were detected in OS xenografts and biopsies, with higher MMP-9 levels in biopsies.
Conclusions:
- MMPs and their inhibitors play a role in the invasive process of human osteosarcoma.
- Targeting MMPs may offer a therapeutic strategy for OS.