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Updated: Feb 4, 2026

Isolation and Culture of Primary Retinal Müller Cells from Sprague-Dawley (SD) Rats
Published on: June 17, 2025
Expression of glycine receptor and transporter on bullfrog retinal Müller cells
Shu-Chen Lee1, Yong-Mei Zhong, Xiong-Li Yang
1Institute of Neurobiology and Institute of Brain Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Abstract:
The expression of the glycine receptor (GlyR) alpha1, alpha2 and beta subunits and glycine transporter (GlyT) on Müller cells was studied in bullfrog retina using double immunofluorescence labeling and confocal scanning microscopy. Double labeling of glial fibrillary acidic protein (GFAP), a specific marker for Müller cells, and the GlyR subunits showed that almost all Müller cells moderately expressed GlyR alpha1 and weakly GlyR beta, whereas no immunoreactivity for GlyR alpha2 was observed. The labeling for GlyR alpha1 and GlyR beta appeared in somata, major processes, endfeet and branchlets of the Müller cells. Müller cells were also GlyT1-labeled. Consistent with previous electrophysiological results, these findings suggest that Müller cells may be involved in modulation of glycinergic transmission by reciprocal interactions with retinal neurons through GlyR and GlyT.
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