Related Experiment Video
Updated: Aug 16, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella
Hong Lin1, Edwin L Civerolo, Rong Hu
1Crop Diseases, Pests & Genetics, U.S. Department of Agriculture-ARS, 9611 S. Riverbend Avenue, Parlier, CA 93648, USA. hlin@fresno.ars.usda.gov
Abstract:
A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.
Related Concept Videos
Modern Molecular Taxonomy
Applications of Molecular Taxonomy

