Related Experiment Video
Updated: Aug 16, 2026

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
Specificity of a Bacteroides thetaiotaomicron marker for human feces
C Andrew Carson1, Jessica M Christiansen, Helen Yampara-Iquise
1Dept. of Veterinary Pathobiology, 201 Connaway Hall, University of Missouri, Columbia, MO 65211, USA. carsonc@missouri.edu
Abstract:
A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.
Related Concept Videos
Bacterial Phylum Bacteroidota
Microbiota of the Large Intestine
Bacterial Gastroenteritis
Bacterial Phylum Tenericutes
