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Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
Published on: June 10, 2020
Identification of Chlamydia trachomatis with polymerase chain reaction in middle ear fluid in otitis media with
Erol Keles1, Yasemin Bulut, Irfan Kaygusuz
1University Medical Faculty Otorhinolaryngology, Department Elazig, Turkey. keleserol@yahoo.com
Abstract:
In this study, 78 middle ear aspiration samples from 59 children between the ages of 2 14 who have undergone myringotomy under general anesthesia were included. The presence of DNA genome of Chlamydia trachomatis was analyzed with polymerase chain reaction in the aspiration material. The relationship between the nature of the effusion fluid and the presence of Chlamydia trachomatis was investigated. Of the seventy eight middle ear effusions, 26 (33.3%) were serous, 31 were (39.7%) mucoid and 21 (26.9%) were sero mucoid in nature. Chlamydia trachomatis DNA genome was identified in seven (8.9%) effusion samples. Of these, three were mucoid (42.8%), three were serous (42.8%) and one was sero mucoid (14.2%) in character. We think that during the bacteriological analyses carried out for the cases of otitis media with effusion, Chlamydia trachomatis should be taken into consideration both at the time of diagnosis and the treatment.
Insights
Chlamydia trachomatis DNA was detected in 8.9% of pediatric middle ear effusion samples. This finding suggests considering Chlamydia trachomatis in otitis media diagnosis and treatment for children.
Area of Science:
- Pediatric Otolaryngology
- Infectious Diseases
- Molecular Diagnostics
Background:
- Otitis media with effusion (OME) is common in children.
- Identifying causative pathogens is crucial for effective treatment.
Purpose of the Study:
- To investigate the prevalence of Chlamydia trachomatis in middle ear effusions of children undergoing myringotomy.
- To explore the association between effusion type and Chlamydia trachomatis presence.
Main Methods:
- Analysis of 78 middle ear aspiration samples from 59 children (ages 2-14).
- Polymerase chain reaction (PCR) used to detect Chlamydia trachomatis DNA.
- Classification of effusion fluid into serous, mucoid, and sero-mucoid types.
Main Results:
- Chlamydia trachomatis DNA was identified in 7 (8.9%) of the 78 effusion samples.
- The positive samples included 3 serous, 3 mucoid, and 1 sero-mucoid effusion.
- No significant association was found between effusion type and Chlamydia trachomatis detection.
Conclusions:
- Chlamydia trachomatis should be considered in the etiological diagnosis of otitis media with effusion in children.
- Testing for Chlamydia trachomatis may be warranted in pediatric OME cases.
- Inclusion of Chlamydia trachomatis in treatment protocols for OME should be evaluated.
