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Updated: Aug 16, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
[Effects of low molecular weight heparin on vascular endothelial growth factor expression of early diabetic
Liang-ying Gan1, Zhong-yuan Yu, Mei-shun Cai
1Department of Nephrology, Peking University People's Hospital, Beijing 100044, China.
Objective:
To investigate the effects of low molecular weight heparin (LMWH) on vascular endothelial growth factor (VEGF) expression of early diabetic nephropathy.
Methods:
Ninety-five male SD rats were randomly divided into three groups: normal control rats, STZ-induced diabetic rats and diabetic rats treated with LMWH. The renal tissues were subjected to immunohistochemical staining after 1, 2, 4, 6, and 8 weeks' treatment respectively to quantify the VEGF expression.
Results:
Immunohistochemical staining demonstrated an increasing in VEGF positive cells in diabetic rats. It was found that there were significant differences in VEGF staining intensity between diabetic rats and normal control rats and between LMWH treated rats and untreated diabetic rats after two weeks treatment.
Conclusion:
The inhibition of VEGF expression may be one of the mechanisms of LMWH's renal protective effects on early diabetic nephropathy.
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