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Updated: Aug 16, 2026
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
Published on: April 9, 2015
[Hypoxia/reoxygenation induced endoplasmic reticulum stress in cultured neonatal rat cardiomyocyte]
Qing-bian Ma1, Wei Gao, Yan-hong Guo
1Department of Cardiology, Peking University Third Hospital, Beijing 100083, China.
Objective:
To investigate the effect of hypoxia/reoxygenation on endoplasmic reticulum stress in cultured neonatal rat cardiomyocytes.
Methods:
Neonatal rat cardiac myocytes in primary culture were exposed to hypoxia for 5.5 hours and subsequently reoxygenation for 2-24 hours. Western blot and RT-PCR were applied to monitor the expression change of GRP78 (glucose regulated protein 78). 2-deoxy-D-glucose (2-DG) was the positive control of this study. Then Western blot and RT-PCR were used to examine the expression of GRP78.
Results:
Cell viability was decreased obviously after hypoxia/reoxygenation. Compared with untreated cells, the GRP78 content of the cells had increased significantly in the hypoxia/reoxygenation cells. The level of GRP78 protein and mRNA elevated from the points of 2 hours to 24 hours after reoxygenation, and increased most obviously at the point of 4 hours after reoxygenation. (4 hours: protein level 142% of the control, mRNA level 200%). 2-DG could induce the increasing expression of GRP78 in a concentration-dependent manner from 10-50 mmol/L.
Conclusion:
Hypoxia/reperfusion can induce endoplasmic reticulum stress in rat cardiomyocytes.
