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Updated: Aug 16, 2026
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Published on: March 5, 2019
Simultaneous detection of double-stranded RNA-induced protein kinase and its specific mRNA by single cell analysis
Mónica M Lozano1, Tanya L Chantawansri, Pao C Chau
1Chemical Engineering Program, University of California La Jolla, San Diego, 92093, USA.
Abstract:
Fluorescent in situ hybridization was combined with flow cytometry to detect the expression of the double-stranded-RNA-induced protein kinase (PKR) in single cells. Labeled anti-sense oligonucleotide was used to target the specific mRNA while the protein was targeted with an antibody. It was demonstrated that the PKR-mRNA signal could be protected through a lengthy immunostaining procedure. The expression pattern of the PKR-mRNA with respect to DNA content was shown to be comparable to that of 18S ribosomal RNA.
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