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Updated: Aug 16, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides
J A Trelles1, A L Valino, V Runza
1Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
Abstract:
Modified nucleosides can be prepared by microbial transglycosylation from cheaper nucleoside precursors using free or immobilised whole cells. An efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as 6-chloro-, 6-methoxy-, 6-iodo- and 6-mercaptopurine ribonucleoside. Out of 100 microorganisms screened, Bacillus stearothermophilus ATCC 12980 was the best for this purpose.
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