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Related Experiment Videos

The origin recognition core complex regulates dendrite and spine development in postmitotic neurons.

Zhen Huang1, Keling Zang, Louis F Reichardt

  • 1Department of Physiology, University of California San Francisco, San Francisco, CA 94143, USA. zhenh@itsa.ucsf.edu

The Journal of Cell Biology
|August 10, 2005
PubMed
Summary

The origin recognition complex (ORC) plays a key role in DNA replication. New findings show ORC subunits are vital for neuronal development, regulating dendrite and dendritic spine formation in the nervous system.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • The origin recognition complex (ORC) is essential for initiating DNA replication.
  • Emerging evidence suggests ORC may have roles beyond DNA replication, including cell division and genome distribution.
  • The expression and function of ORC in the nervous system are not well understood.

Purpose of the Study:

  • To investigate the role of ORC core subunits in the nervous system.
  • To determine the localization and function of ORC in neuronal development.

Main Methods:

  • Analysis of ORC subunit expression in the nervous system.
  • Subcellular localization studies of ORC.
  • siRNA-mediated knockdown of ORC subunits in neurons.

Related Experiment Videos

  • Expression of ORC ATPase motif mutants.

    • Main Results:

    • ORC core subunits are highly expressed in the nervous system.
    • ORC subunits are localized to the neuronal somatodendritic compartment and membrane fraction.
    • ORC subunit knockdown significantly reduced dendritic branching and spine formation.
    • Expression of ORC ATPase mutants enhanced dendritic arborization.

    Conclusions:

    • The ORC core complex has a novel function in regulating dendrite and dendritic spine development in postmitotic neurons.
    • ORC's role extends beyond DNA replication to neuronal morphogenesis.