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Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
Published on: February 21, 2014
Layer by layer self-assembled polyelectrolyte multilayers with embedded phospholipid vesicles obtained by spraying:
M Michel1, A Izquierdo, G Decher
1Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France.
Langmuir : the ACS Journal of Surfaces and Colloids
|August 11, 2005
Summary
This study demonstrates that phospholipid vesicles remain intact when incorporated into sprayed polyelectrolyte multilayers. These vesicle-containing films can serve as reservoirs for controlled release applications.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Nanotechnology
Background:
- Phospholipid vesicles can be incorporated into poly(glutamic-acid)/poly(allylamine) multilayered polyelectrolyte films.
- Previous work suggested vesicles remain intact when adhering to surfaces in these films.
Purpose of the Study:
- To investigate the incorporation of phospholipid vesicles into polyelectrolyte multilayers using a spraying technique.
- To confirm vesicle integrity within sprayed multilayers and explore multilayer construction with multiple vesicle layers.
Main Methods:
- Fabrication of polyelectrolyte multilayers (PEM) via alternating spraying of polyanion (PGA) and polycation (PAH) solutions with phospholipid vesicles.
- Characterization of vesicle integrity using cyclic voltammetry with ferrocyanide-loaded vesicles.
- Construction and analysis of PEMs with multiple, separated vesicle layers.
Main Results:
- Spraying technique successfully incorporates intact phospholipid vesicles into PGA/PAH multilayer films.
- Cyclic voltammetry confirmed vesicle integrity within the sprayed multilayers.
- Multilayers with two distinct vesicle layers separated by polyelectrolyte bilayers were successfully constructed.
Conclusions:
- Sprayed polyelectrolyte multilayers effectively encapsulate intact phospholipid vesicles.
- These functionalized multilayers hold potential as reservoirs for controlled release of drugs or bioactive molecules.
- The developed method offers a versatile platform for creating advanced bioactive coatings.
