Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation and analysis of 12,000 AI-driven CAR-T designs in the <i>Bits to Binders</i> competitions.

bioRxiv : the preprint server for biology·2026
Same author

Epigenome-wide association study of nuclear DNA methylation in relation to mitochondrial heteroplasmy.

Nature communications·2025
Same author

Vascular Endothelial Growth Factor B Modulates Cardiac Functions via Ferroptosis Pathways in Post-Myocardial Infarction.

Cells·2025
Same author

Increasing <i>SOX10</i> Expression Overcomes Schizophrenia-Associated Early Oligodendrocyte Growth Deficits In Vitro.

Biological psychiatry global open science·2025
Same author

Nondestructive Larval Genotyping of <i>Danio rerio</i> for Mitochondrial and Nuclear DNA Genetics.

Zebrafish·2025
Same author

Methyl-CpG-binding domain as a protein interaction partner in promoter regulation and neurodevelopment through evolutionary expanded entanglement.

American journal of physiology. Cell physiology·2025

Related Experiment Video

Updated: Jan 18, 2026

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
07:32

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Published on: April 29, 2021

5.9K

Functional analysis of human hematopoietic stem cell gene expression using zebrafish.

Craig E Eckfeldt1, Eric M Mendenhall, Catherine M Flynn

  • 1Division of Hematology, Oncology, and Transplantation, Department of Medicine, Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA.

Plos Biology
|August 11, 2005
PubMed
Summary

Researchers identified novel genes regulating hematopoietic stem cell (HSC) fate by comparing human cell transcriptomes and screening zebrafish. This approach revealed 14 key genes essential for blood development, advancing our understanding of hematopoiesis.

More Related Videos

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos
10:28

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

8.3K
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

11.4K

Related Experiment Videos

Last Updated: Jan 18, 2026

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
07:32

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Published on: April 29, 2021

5.9K
Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos
10:28

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

8.3K
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

11.4K

Area of Science:

  • Hematology
  • Developmental Biology
  • Genetics

Background:

  • Hematopoietic stem cell (HSC) transcriptomes are known, but HSC-specific gene functions in hematopoiesis are unclear.
  • Identifying regulators of HSC fate is crucial for understanding blood development and related disorders.

Purpose of the Study:

  • To identify novel genes involved in hematopoietic stem cell (HSC) fate decisions.
  • To functionally validate candidate HSC-specific genes using a high-throughput zebrafish screen.

Main Methods:

  • Compared transcriptomes of human umbilical cord blood and bone marrow stem/progenitor cells.
  • Performed a morpholino antisense oligonucleotide (MO)-based functional screen in zebrafish for 61 candidate genes.
  • Validated findings using quantitative RT-PCR, secondary MOs, and cDNA rescue experiments.

Main Results:

  • Identified 277 differentially expressed transcripts conserved across human cell sources.
  • Functional screening in zebrafish revealed 14/61 (23%) genes caused hematopoietic defects upon knockdown.
  • Specific defects in HSC specification, proliferation, and/or differentiation were confirmed for zebrafish spry4.

Conclusions:

  • This study presents a successful high-throughput functional screen for validating HSC-specific genes using a zebrafish model.
  • The identified genes represent critical regulators of hematopoiesis and offer new insights into HSC fate decisions.