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Relationship of serum interleukin-7 concentration and the coagulation state in children with nephrotic syndrome
Anna Wasilewska1, Walentyna M Zoch-Zwierz, Barbara Tomaszewska
1Department of Pediatrics, Medical University in Bialystok, Bialystok, Poland. annwasil@interia.pl
Insights
Interleukin-7 (IL-7) levels are elevated in children with nephrotic syndrome (NS), correlating with increased platelet counts and hemostatic factors. Treatment with prednisone reduces IL-7 levels in NS patients.
Area of Science:
- Pediatric Nephrology
- Hematology
- Immunology
Background:
- Nephrotic syndrome (NS) is associated with hypercoagulable state due to enhanced platelet reactivity.
- Thrombocytosis and platelet aggregation in NS can lead to the release of cytokines like interleukin-7 (IL-7).
Purpose of the Study:
- To evaluate serum IL-7 levels in children diagnosed with NS.
- To determine the correlation between IL-7 concentration and platelet count, hemostatic factors, and NS severity.
Main Methods:
- A study involving 26 children with NS and 20 healthy controls.
- Serum IL-7 levels were measured using enzyme-linked immunosorbent assay (ELISA) before and during prednisone treatment for NS patients.
Main Results:
- Children with NS exhibited significantly higher serum IL-7 levels compared to controls (P < 0.01).
- IL-7 levels decreased to normal ranges after prednisone treatment.
- A positive correlation was found between IL-7 and platelet count/fibrinogen, and a negative correlation with antithrombin III.
Conclusions:
- Serum IL-7 levels increase in children with NS, correlating with elevated platelet counts and other hemostatic factors.
- IL-7 levels normalize with effective NS treatment, suggesting its role in the syndrome's hypercoagulable state.
Background:
Enhanced platelet reactivity may play a significant role in the hypercoagulable state of nephrotic syndrome (NS). Thrombocytosis with platelet aggregation cause the release of some cytokines, among them interleukin-7 (IL-7). The aim of the study was to evaluate serum IL-7 levels in children with the symptoms of NS and to determine a correlation between its concentration and platelet count, other hemostatic factors, and NS intensity.
Methods:
The study was performed in two groups. I--the examined group of 26 children with NS (12 boys, 14 girls) aged 6.8 +/- 2.1 years, subjected to two examinations: A--before treatment, B--during treatment with prednisone (60 mg/kg 24 h after albuminuria regression); and II--the control group (C) of 20 healthy children. Serum IL-7 level was assayed by enzyme-linked immunosorbent assay method using a R & D Quantikine set.
Results:
In group I, IL-7 level in examination A (33.33 +/- 33.24 pg/mL) was higher than in the control subjects (P < 0.01). In examination B, IL-7 concentration was reduced to the level of 10.86 +/- 5.22 pg/mL and did not differ from the controls (P > 0.05). A positive correlation was observed between IL-7 and platelet count and serum fibrinogen level. A negative correlation was noted with antithrombin III concentration. No correlation was found with serum levels of albumin and cholesterol or urine protein.
Conclusion:
In children with NS, serum IL-7 level increases proportionally to the elevated platelet count and other hemostatic components, but shows no correlation with serum albumin or urine protein.
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