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Updated: Aug 16, 2026
Overexpressing and Purifying a Toxic Nuclease from Escherichia coli
Published on: August 29, 2025
Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus:
Chia-Wei Lai1, Yao-Chi Chung, Yiu-Kay Lai
1Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu, Taiwan.
Abstract:
Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.
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