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Updated: Aug 16, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter]
Jiu-zeng Dai1, Jun Cheng, Jian-hui Qu
1Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039, China. daijiuzeng18@163.com
Objective:
To investigate the effect of hepatitis C virus (HCV) non-structure protein NS5A on the activity of calcium-regulating protein alpha subunit of nascent polypeptide-associated complex (NACA) promoter.
Methods:
HepG2 cell plasmid pCAT3-NACA, containing NACA promoter, was transfected alone or cotransfected with pcDNA3.1(-)-NS5A, and chloramphenicol acetyl transferase (CAT) enzyme activity was assayed by enzyme-linked immunoassay (ELISA).
Results:
The CAT activity in the pcDNA3.1(-)-NS5A cotransfection group was 20.7% of the CAT activity in the pCAT3-NACA group.
Conclusion:
HCV non-structural protein NS5A has a down-regulating effect on the promoter of NACA gene.
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