Related Experiment Video
Updated: Aug 16, 2026

Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Comparison of real-time PCR and pyrosequencing for typing Bordetella pertussis toxin subunit 1 variants
Martin Storm1, Abdolreza Advani, Monica Pettersson
1Uppsala University Hospital, Department of Medical Sciences, Clinical Bacteriology, Uppsala, Sweden.
Abstract:
We describe two newly developed methods for rapid typing of the pertussis toxin subunit 1 gene (ptxS1). A real-time PCR assay based on hybridization probes and a Pyrosequencing assay were developed and the specificity, sensitivity, cost, hands-on time and post-assay data processing were compared to Sanger sequencing. Both methods enabled discrimination of all four allelic variants, correctly identified all ptxS1 alleles of 143 strains tested and proved suitable for large-scale screening of B. pertussis strains.
More Related Videos
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Modern Molecular Taxonomy

