Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Efficiency of nano-electrospray ionization.

Ayman El-Faramawy1, K W Michael Siu, Bruce A Thomson

  • 1Department of Chemistry and Center for Research in Mass Spectrometry, York University, Toronto, Ontario, Canada.

Journal of the American Society for Mass Spectrometry
|August 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Cerebrospinal Fluid Proteome of Alzheimer's Disease Patients: Differentiation and Prediction of Responders and Non-Responders to Lymphatic-Venous Anastomosis.

Analytical chemistry·2026
Same author

Spectronaut and DIA-NN: A Comparison of their Performance in the Analysis of Lung Adenocarcinoma Biopsies.

ACS omega·2026
Same author

Synergism between Distribution, Mechanistic and Omics Analyses in Studies on Assessing Environmental Exposure and Human Health Impact.

Environment & health (Washington, D.C.)·2026
Same author

<i>Environment & Health</i> Best Paper and Outstanding Reviewer Awards 2023-2024.

Environment & health (Washington, D.C.)·2025
Same author

Understanding the Binding and Structures in Model Complexes of Polypeptides and Cofactors.

ACS physical chemistry Au·2025
Same author

Cerebrospinal Fluid p‑Tau181/Amyloid Beta 42 Ratio Identifies Lymphatic-Venous Anastomosis Patients Who Respond to and Benefit from the Surgery for Relief of Cognitive Impairment with a Diagnostic Accuracy of 0.744.

ACS omega·2025

Nano-electrospray ionization efficiency was measured for various analytes. Lower flow rates generally improved efficiency, with some analytes reaching up to 12% efficiency.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Ionization Techniques

Background:

  • Nano-electrospray ionization (nESI) is a crucial technique in mass spectrometry.
  • Understanding nESI efficiency is vital for quantitative analysis and sensitivity.
  • Previous studies have explored factors influencing nESI performance, but comprehensive efficiency measurements across different analytes and flow rates are needed.

Purpose of the Study:

  • To quantitatively measure the efficiency of nano-electrospray ionization (nESI).
  • To determine the optimal flow rate for maximizing nESI efficiency.
  • To investigate variations in nESI efficiency among different analytes and emitters.

Main Methods:

  • nESI experiments were conducted using a triple-quadrupole mass spectrometer.

Related Experiment Videos

  • Efficiency was defined as the ratio of ions detected to ions emitted from the needle.
  • Experiments involved varying the flow rate of analytes including dodecyltrimethyl ammonium (DDTMA) bromide, myoglobin, Glu-[1]-fibrinopeptide, and gramicidin S.

    • Main Results:

    • nESI efficiency generally increased with decreasing flow rate.
    • Observed efficiencies ranged from approximately 1% to a maximum of 12% for DDTMA.
    • Significant needle-to-needle variations in efficiency were noted, attributed to ion generation differences.

    Conclusions:

    • Optimizing flow rate is critical for maximizing nESI efficiency.
    • Analyte type and emitter characteristics significantly impact ionization efficiency.
    • Further research into controlling ion generation is necessary for reproducible nESI performance.