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Related Experiment Videos

Ursodeoxycholic acid and superoxide anion.

Predrag Ljubuncic1, Omar Abu-Salach, Arieh Bomzon

  • 1Department of Pharmacology, Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Efron Street, PO Box 9649, Haifa 31096, Israel.

World Journal of Gastroenterology
|August 13, 2005
PubMed
Summary

Ursodeoxycholic acid (UDCA) can scavenge superoxide anion (O(2)(-)), a reactive oxygen species. This antioxidant activity may offer therapeutic benefits for patients with primary biliary cirrhosis.

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Area of Science:

  • Biochemistry
  • Pharmacology
  • Hepatology

Background:

  • Reactive oxygen species, such as superoxide anion (O(2)(-)), play a role in liver disease pathogenesis.
  • Ursodeoxycholic acid (UDCA) is a bile acid with known therapeutic effects in cholestatic liver diseases.

Purpose of the Study:

  • To investigate the antioxidant capacity of ursodeoxycholic acid (UDCA).
  • To determine if UDCA can directly scavenge superoxide anion (O(2)(-)).

Main Methods:

  • A cell-free system using xanthine-xanthine oxidase (X-XO) was employed to generate superoxide anion (O(2)(-)).
  • The ability of UDCA to scavenge O(2)(-) was assessed.
  • The effect of UDCA on O(2)(-)-induced ascorbic acid (AA) oxidation was measured in hepatic post-mitochondrial supernatants.

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Main Results:

  • Ursodeoxycholic acid (UDCA) demonstrated the ability to scavenge superoxide anion (O(2)(-)) at concentrations of 0.5 and 1 mmol/L.
  • UDCA did not inhibit O(2)(-) generation by the X-XO system.
  • At 100 micromol/L, UDCA reduced the rate of O(2)(-)-induced ascorbic acid oxidation.

Conclusions:

  • Ursodeoxycholic acid (UDCA) possesses direct superoxide anion (O(2)(-)) scavenging properties.
  • The antioxidant activity of UDCA may contribute to its beneficial effects in primary biliary cirrhosis.