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Related Experiment Videos

[Determination of ascorbic acid by indirect fluorimetry].

Chang-lun Tong1, Guang-hong Xiang, Wei-ping Liu

  • 1Institute of Environmental Science, Zhejiang University, Hangzhou 310029, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|August 16, 2005
PubMed
Summary

Ascorbic acid (vitamin C) can be detected using a sensitive fluorescence method. This technique measures the enhanced fluorescence of cerium(III) ions produced by ascorbic acid

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Context:

  • Cerium(IV) ions lack fluorescence, but reduction to cerium(III) ions by ascorbic acid induces characteristic fluorescence.
  • Sodium hexametaphosphate significantly enhances the fluorescence intensity of the cerium(III) system.

Purpose:

  • To develop a sensitive, indirect method for determining ascorbic acid concentrations.
  • To utilize the fluorescence enhancement of cerium(III) ions for quantitative analysis.

Summary:

  • An indirect fluorescence method was developed by monitoring the cerium(IV) to cerium(III) reduction by ascorbic acid.
  • Measurements were performed using excitation and emission wavelengths of 303 nm and 340 nm, respectively.
  • A linear relationship was observed between fluorescence intensity and ascorbic acid concentration (1.0 x 10(-7)-8.0 x 10(-6) mol x L(-1)) with a detection limit of 1.6 x 10(-8) mol x L(-1).

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Impact:

  • Provides a highly sensitive analytical method for quantifying ascorbic acid.
  • Successfully applied to real-world samples, including vitamin C tablets.
  • Demonstrates the utility of cerium fluorescence in biochemical analysis.