Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondrial calcium signalling in cell death.

Sara Leo1, Katiuscia Bianchi, Marisa Brini

  • 1Department of Experimental and Diagnostic Medicine, Section of General Pathology, and Interdisciplinary Center for the Study of Inflammation (ICSI), University of Ferrara, Italy.

The FEBS Journal
|August 16, 2005
PubMed
Summary

Targeted probes enable precise measurement of intracellular calcium ([Ca2+]), revealing rapid mitochondrial matrix accumulation during cell stimulation. This impacts cellular metabolism and cell death pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same author

Dissecting mitochondria-nucleus contact sites using SPLICS reveals a distributed and dynamic tethering network.

Biology direct·2026
Same author

Mitochondrial calcium uptake drives organelle remodeling to promote inflammasome-dependent cytokine release.

Cell death and differentiation·2026
Same author

Development and implementation of low-cost, grocery-sourced oral immunotherapy protocols.

The journal of allergy and clinical immunology. In practice·2026
Same author

Melatonin and mitochondrial protection in cardiac ischemia-reperfusion injury: mechanisms, evidence and translational perspectives.

Basic research in cardiology·2026
Same author

A novel inhibitor of soluble epoxide hydrolase that adducts C521 is cardioprotective.

Redox biology·2025

Area of Science:

  • Cellular biology
  • Biochemistry
  • Molecular imaging

Background:

  • Development of targeted luminescent/fluorescent protein probes for precise intracellular calcium ([Ca2+]) measurement.
  • Enabled novel insights into cellular calcium homeostasis across different compartments.

Discussion:

  • Mitochondrial calcium ([Ca2+]) uptake kinetics upon physiological cell stimulation.
  • Role of mitochondrial calcium in regulating aerobic metabolism and energy production.
  • Involvement of mitochondrial calcium in the induction of programmed cell death pathways.

Key Insights:

  • Demonstrated rapid calcium ([Ca2+]) accumulation in the mitochondrial matrix.
  • Highlighted the critical role of mitochondrial calcium in cellular energy metabolism.

Related Experiment Videos

  • Established the link between mitochondrial calcium and cell death induction.

    • Outlook:

    • Further elucidation of in vivo regulatory mechanisms governing mitochondrial calcium.
    • Exploring therapeutic strategies targeting mitochondrial calcium handling.
    • Investigating the broader implications of mitochondrial calcium in disease pathogenesis.