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Updated: Aug 16, 2026
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
Published on: November 29, 2024
Platelet release of beta-thromboglobulin and platelet factor 4 and serotonin in plasma samples
Ryunosuke Ohkawa1, Yuji Hirowatari, Kazuhiro Nakamura
1Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
Objectives:
Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated.
Methods:
Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method.
Results:
Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin.
Conclusions:
Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
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