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Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Mucosal and systemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens
Analia Berinstein1, Cecilia Vazquez-Rovere, Sebastian Asurmendi
1Instituto de Biotecnología, CICVyA, INTA, Cc25 B1712WAA, Castelar, Buenos Aires, Argentina. aberinst@cicv.inta.gov.ar
Abstract:
As a step towards developing a safe and effective edible vaccine against Newcastle disease virus (NDV), we have explored the use of plants genetically engineered to express viral proteins. We report the construction of transgenic potato plants expressing the genes coding for immunogenic proteins of NDV under the regulation of CaMV 35S promoter and its immunogenicity in mice. All mice receiving transgenic plant extracts in incomplete Freund adjuvant produced specific anti-NDV antibodies. Animals fed with transgenic leaves showed a specific response against NDV. Detection of IgA released from in vitro-cultured intestinal tissue fragments indicated the presence of IgA-secreting cells in the gut.
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