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Related Experiment Videos

[Decrease of mitochondrial sensitivity to Ca2+-induced pore opening during long-term incubation].

O V Akopova, V F Sagach

    Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
    |August 17, 2005
    PubMed
    Summary

    Mitochondria from rat hearts are highly sensitive to calcium-induced permeability transition pore opening shortly after isolation. This sensitivity decreases over time, correlating with changes in the calcium uniporter kinetics.

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    MITOCHONDRIAL DYSFUNCTION IN THE AGING HEART IS ACCOMPANIED BY CONSTITUTIVE NO-SYNTHASES UNCOUPLING ON THE BACKGROUND OF OXIDATIVE AND NITROSATIVE STRESS.

    Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018

    Area of Science:

    • Mitochondrial physiology
    • Cardiovascular research
    • Calcium signaling

    Background:

    • Mitochondria play a crucial role in cellular energy production and calcium homeostasis.
    • The mitochondrial permeability transition pore (PTP) is a key regulator of cell death and survival.
    • Calcium overload is implicated in various cardiac pathologies.

    Purpose of the Study:

    • To investigate the time-dependent changes in calcium sensitivity of mitochondrial permeability transition pore (PTP) in isolated rat myocardium.
    • To determine the kinetic parameters of Ca2+-dependent PTP activation and their correlation with mitochondrial sensitivity.

    Main Methods:

    • Isolation of mitochondria from rat myocardium.
    • Measurement of calcium-induced PTP opening.

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  • Determination of threshold calcium concentrations and apparent K(Ca) values.
  • Kinetic analysis of Ca2+-uniporter activity over time.

    • Main Results:

    • Isolated rat myocardial mitochondria show high sensitivity to Ca2+-induced PTP opening within one hour post-isolation (threshold Ca2+ 25-50 nmol/mg protein, K(Ca) 40 microM).
    • Incubation for 2-4 hours post-isolation leads to a decrease in mitochondrial sensitivity to PTP opening.
    • This decreased sensitivity correlates with an increase in apparent K(Ca) values (up to 0.4 microM).

    Conclusions:

    • Mitochondrial sensitivity to Ca2+-induced PTP opening is highest shortly after isolation and diminishes over time.
    • Changes in the kinetic parameters of the Ca2+-uniporter are associated with altered mitochondrial sensitivity to calcium-induced permeability transition.