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Analytical Techniques for Assaying Nitric Oxide Bioactivity
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Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Identification of nitric oxide donors by biomimetic HTS application

György T Balogh1, Balázs Dalmadi, Attila Bielik

  • 1Pilot Plant II, Gedeon Richter Ltd., P.O. Box 27, H-1475, Budapest, Hungary.

Combinatorial Chemistry & High Throughput Screening
|August 17, 2005
PubMed

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Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...