Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method for optimizing multiplex DNA microsatellite analysis in low quality archival specimens.

Irina Drobinskaya1, Helmut Erich Gabbert, Gabriela Moeslein

  • 1Department of Surgery, Heinrich-Heine-University of Duesseldorf, Duesseldorf, Germany.

Anticancer Research
|August 17, 2005
PubMed
Summary

This study introduces a new method for microsatellite instability testing in colorectal cancer, improving DNA amplification from archival tissue. The approach enhances diagnostic accuracy for hereditary non-polyposis colorectal cancer (HNPCC) screening.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep-learning enabled combined measurement of tumour cell density and tumour infiltrating lymphocyte density as a prognostic biomarker in colorectal cancer.

BJC reports·2025
Same author

Transformer-based biomarker prediction from colorectal cancer histology: A large-scale multicentric study.

Cancer cell·2023
Same author

Diversity bias in colorectal surgery: a global perspective.

Updates in surgery·2022
Same author

Commentary on the manuscript "Women surgeons in Italy": drawbacks and opportunities. Results from a nationwide interdisciplinary survey by women in surgery Italia.

Updates in surgery·2022
Same author

Adenoma and colorectal cancer risks in Lynch syndrome, Lynch-like syndrome and familial colorectal cancer type X.

International journal of cancer·2021
Same author

A Snapshot of the International Views of the Treatment of Rectal Cancer Patients, a Multi-regional Survey: International Tendencies in Rectal Cancer.

World journal of surgery·2020

Area of Science:

  • Molecular biology
  • Genetics
  • Oncology

Background:

  • Microsatellite instability (MSI) testing is crucial for screening hereditary non-polyposis colorectal cancer (HNPCC).
  • Investigating MSI in formalin-fixed paraffin-embedded (FFPE) tissues is challenging due to DNA degradation.

Purpose of the Study:

  • To develop an improved method for MSI analysis in archival FFPE colorectal cancer specimens.
  • To enhance the amplification of Bethesda panel loci from low-quality DNA.

Main Methods:

  • A novel multiplex PCR approach utilizing touch-up cycling conditions was developed.
  • The method focuses on specific amplification of Bethesda reference panel loci.

Main Results:

  • The new method successfully analyzed MSI in 89% (75/84) of tumors.

Related Experiment Videos

  • Standard PCR protocols failed in 36% of cases, highlighting the new method's efficacy.

    • Conclusions:

    • The developed methodical approach is recommended for analyzing archival FFPE tissues.
    • This method provides efficient and accurate amplification of MSI markers, regardless of DNA quality.