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A method for cytogenetic analysis of boar spermatozoa using hamster oocytes
M Benkhalifa1, M Bonneau, P Popescu
1Laboratoire de Cytogénétique Médicale, Faculté de Médecine, Clermont-Ferrand, France.
Annales De Genetique
|January 1, 1992
Abstract:
In this paper, the authors detail a method for displaying boar spermatozoa chromosomes using heterospecific zona-free hamster oocyte penetration technique. Semen samples from two Large-White boars having a normal spermogram were studied. The first one had a normal karyotype (38,XY), the second carried a reciprocal translocation rcp(3;7)(p1,3;q2,1). After in vitro fertilization by capacitated sperm, culture and cytogenetic analysis of hamster eggs we obtained metaphase spreads of spermatozoa chromosomes. The ratio of X- and Y-bearing spermatozoa was 49.2% and 50.8%, respectively.