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Published on: May 5, 2023
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2
Vincenzo Rivieccio1, Riccardo Mannini, Luigi Concilio
1Istituto di Genetica e Biofisica A. Buzzati Traverso, Consiglio Nazionale delle Ricerche, Italy.
Abstract:
The Krüppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship.

