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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
Published on: March 6, 2013
Influence of disulfide bonds on the conformational changes and activities of refolded phytase
1College of Life Science, Shandong Agricultural University, Shandong Tai'an 271018, People's Republic of China.
Protein and Peptide Letters
|August 17, 2005
Abstract:
Aspergillus sp. phytase contains five disulfide bonds. In order to elucidate their role, the reactivation and refolding of phytase in the absence and presence of dithiothreitol (DTT) was investigated. The results indicated that the disulfide bonds play an important role in the catalytic activity and conformational stability of the enzyme.
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