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Routine Screening Method for Microparticles in Platelet Transfusions
Published on: January 31, 2018
Quantitation of microparticles released from coated-platelets
G L Dale1, G Remenyi, P Friese
1Department of Medicine, W.K. Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. george-dale@ouhsc.edu
Journal of Thrombosis and Haemostasis : JTH
|August 17, 2005
Summary
Dual agonist stimulation of platelets generates coated-platelets that release microparticles. These microparticles express specific surface markers and their release is enhanced by compounds affecting the mitochondrial permeability transition pore.
Area of Science:
- Hematology
- Cell Biology
- Biochemistry
Background:
- Coated-platelets are a sub-population of platelets generated by dual stimulation with thrombin and convulxin.
- These cells retain procoagulant proteins and express phosphatidylserine (PS).
Purpose of the Study:
- To investigate the release of microparticles from coated-platelets using a novel methodology.
- To characterize the surface markers and release kinetics of these microparticles.
Main Methods:
- Platelets were labeled with Bodipy-maleimide and stimulated with convulxin plus thrombin.
- Fluorescence confocal microscopy and flow cytometry were employed to detect and quantify microparticles.
- Analysis included assessing surface markers like glycoprotein IIb/IIIa, glycoprotein Ib, CD9, and PS.
Main Results:
- Dual agonist stimulation (thrombin and convulxin) induced the release of microparticles (0.3-0.5 microm) from coated-platelets.
- Microparticles were positive for glycoprotein IIb/IIIa, glycoprotein Ib, CD9, and PS, but negative for fibrinogen and thrombospondin.
- Activation produced significantly more microparticles (15 +/- 5 per coated-platelet) compared to single agonist stimulation. Phenylarsine oxide and diamide enhanced microparticle release.
Conclusions:
- Coated-platelets, generated by dual agonist stimulation, release phosphatidylserine-exposing microparticles.
- The release of these microparticles is dependent on dual activation and can be modulated by agents affecting the mitochondrial permeability transition pore.
